Here we present a method for the fabrication of microarrays in which presynthesized oligonucleotides are ejected onto a glass surface by a Bubble Jet printing device. The oligonucleotides are then ...

Oligonucleotide microarray (DNA chip)–based hybridization analysis is a promising new technology which potentially allows rapid and cost–effective screens for all possible mutations and sequence ...

We compared the applicability of an enhanced chemiluminescent (ECL) method for using gene probes with that of radioactive probes to identify enterotoxigenic Escherichia coli (ETEC) in stools of ...

Therapeutic oligonucleotides act on different stages of pathological gene expression. Standard schematic of oligonucleotide activities in pathogenesis disease progression. Decoys bind to transcription ...

BHQplus Probes form more stable probe-target duplexes, enabling shorter oligonucleotide designs. Shorter oligos offer high specificity and better detection of difficult targets, such as AT-rich ...

The ability to label nucleic acids with a variety of tagged molecules has led to astonishing advances in molecular biology. Labeled DNA, RNA, and oligonucleotide probes have made possible techniques ...

About nonradioactive nucleic acid detection -- Isolation of DNA from plants -- Isolation of high-molecular-weight DNA from animal cells -- Restriction enzyme digestion, gel electrophoresis and vacuum ...

High-resolution melting has proven to be a highly sensitive method for mutation discovery. PCR is performed in the presence of the double-strand DNA binding dye LCGreen® Plus. Samples are then ...

PCR has fundamentally changed molecular biology, offering a rapid means to amplify minute quantities of DNA. Real-time quantitative PCR (Q-PCR) techniques are designed to facilitate accurate ...

MicroRNAs are a class of noncoding regulatory RNA molecules that affect gene expression by binding to 3´-untranslated regions of messenger RNAs (mRNAs). Although recently identified as a new class of ...

Every graduate student, creative person and inventor of any stripe has experienced periods when nothing seems to come together, some of us many times. I was waist-deep in such pit after a year-long ...