A real-time polymerase chain reaction (real-time PCR, or qPCR when used quantitatively) is a laboratory technique of molecular biology based on the polymerase chain reaction (PCR).

This section provides an overview of real-time PCR, reverse-transcription quantitative PCR techniques, and the choice of instruments that Bio-Rad offers for these techniques.

Aug 14, 2025 · Real-Time PCR is a technique used to monitor the progress of a PCR reaction in real-time. At the same time, a relatively small amount of PCR product (DNA, cDNA or RNA) can be …

Real-time polymerase chain reaction (qPCR) is the ability to monitor the progress of the PCR as it occurs in real time. Data is therefore collected throughout the process, rather than at the end of the …

Dec 16, 2022 · Successful detection of the first SARS-CoV-2 cases using the real-time polymerase chain reaction (real-time PCR) method reflects the power and usefulness of this technique. Real-time …

Real-time PCR, also referred to as quantitative PCR or qPCR, is a sophisticated molecular biology technique designed for the accurate quantification of nucleic acids.

Real-time PCR (qPCR) combines PCR amplification with simultaneous fluorescence monitoring for quantifying target DNA or RNA in real-time. qPCR offers advantages in pathogen diagnostics, …

What is real-time PCR? Real-time PCR, also known as quantitative or qPCR, measures the amount of PCR product present during a given cycle. The amplified DNA product, or amplicon, is detected in an …

Real-time PCR refers to the monitoring of PCR product accumulation at each cycle of amplification and is typically achieved by measuring fluorescence at one or several times during each cycle.

Feb 1, 2025 · Real-time PCR, or qPCR, is a powerful tool for detecting and measuring DNA or RNA with unprecedented accuracy, which, unlike regular PCR, needs no gel electrophoresis for results …